Lipofectamine Mediated Gene Delivery: Cationic Lipid Helps to Overcome the Cell-Surface Barriers in Hepatocytes

Authors

  • Biswanath Patra Daniel Baugh Institute for Functional Genomics/Computational Biology, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 1020 Locust street, 320A JAH, Philadelphia (19107), PA

Keywords:

Lipofectamine, hepatocyte, HEK293, PGL3, GFP plasmid, TEX615

Abstract

The objective of this study is to find effective siRNA delivery vehicle targeting liver, specifically into hepatocytes. Recently developed cationic lipid lipofectamine provide a very high density of positive charges along its backbone and have been reported to be effective in siRNA delivery to target liver cells. We compared lipofectamine mediated siRNA delivery with a cationic polymer based approach (jetPEI), in cultured fresh rat hepatocytes and human embryonic kidney cell line HEK293. We tested plasmids encoding either GFP or luciferase, and a TEX615 red dye labeled oligo to examine efficient translocation into primary hepatocytes in culture. Our results clearly dem­onstrated the higher efficacy of lipofectamine over jetPEI in targeting hepatocytes for both plasmid and labeled oligo delivery.

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Published

2011-09-07

How to Cite

1.
Patra B. Lipofectamine Mediated Gene Delivery: Cationic Lipid Helps to Overcome the Cell-Surface Barriers in Hepatocytes. IJBSM [Internet]. 2011 Sep. 7 [cited 2025 Sep. 21];2(Sep, 3):313-9. Available from: https://www.ojs.pphouse.org/index.php/IJBSM/article/view/162

Issue

Vol. 2 No. Sep, 3 (2011)

Section

Articles

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